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AlerTox ELISA

Introduction

Food allergies represent an important health problem in developed countries. Undeclared allergens as contaminants in food products constitute a high risk to people sensitive to these allergens. In hypersensitive individuals, even the intake of trace amounts of these allergens can cause digestive disorders, respiratory symptoms, skin reactions or life-threatening reactions like anaphylactic shock. Therefore, reliable detection methods are needed to detect these food allergens in order to ensure compliance with European Food Labelling Directive 2003/89/EC and to improve consumer’s safety. Eggs from hens (Gallus gallus) is considered allergenic food and is explicitly mentioned in the European Food Labelling Directive. The egg whit is rich in allergens which are Ovomucoid (Gal d1), Ovalbumin (Gal d2), Ovotransferrin (Conoalbumin) (Gal d3) and Lysozyme (Gal d4). Pure Ovalbumin is often used for the fining of wine there for wine consumption with non declared allergens is a hazard for people suffering from allergies.

Milk is also considered as allergenic food and is explicitly mentioned in the European Food Labelling Directive. The major allergens present in milk are casein and beta lactoglobulin.

 

Test principle

AlerTox kits are quantitative ELISA Sandwich type immunoabsorbent assay designed for the detection and quantification of the allergen en question. During the test, the sample is added to a plate that specifically recognizes the allergen. After the washing steps, the addition of another antibody conjugated to peroxidase (HRP), which also recognizes the allergen, results in a measurable signal.

AlerTox  ELISA Sandwich is a direct method therefore the higher concentration of allergen present the more intense the signal will be.

The ELISA Sandwich is a technique usually used for the analysis of substances found at very low concentrations. This combined with the high specificity and sensitivity of the antibodies used in these test allows this method to precisely quantify allergen in beverages and food. AlerTox ELISA can be used with any type of food sample. However, AlerTox Lysozyme and Ovalbumin kits are only validated for wine samples.

Technical Specifications

(LOD: Limit of Detection, LOQ: Limit of Quantification)

Kit LOD LOQ Quantification Range
AlerTox Coconut 0.4 ppm 2 ppm (25-12.5-5-2-0 ppm)
AlerTox Lupine 0.2 ppm 2 ppm (30-15-5-2-0 ppm)
AlerTox Sesame 0.2 ppm 2 ppm (20-10-4-2-0 ppm)
AlerTox Mustard 1 ppm 2 ppm (50-25-10-2-0 ppm)
AlerTox Macadamia 0.1 ppm 1 ppm (25-12.5-5-1-0 ppm)
AlerTox Cashew 0.2 ppm 2 ppm (50-25-10-2-0 ppm)
AlerTox Soy (STI) 16 ppb 50 ppb (600-300-150-50-0 ppb)
AlerTox Walnut 0.6 ppm 2 ppm (50-15-5-2-0 ppm)
AlerTox Peanut 0.3 ppm 1 ppm (30-15-5-1-0 ppm)
AlerTox Hazelnut 0.3 ppm 1 ppm (40-15-5-1-0 ppm)
AlerTox Almond 0.2 ppm 0.5 ppm (10-5-2-0.5-0 ppm)
AlerTox Pistachio 0.13 ppm 1 ppm (25-12.5-5-1-0 ppm)
AlerTox Egg 0.05 ppm 0.4 ppm (8-4-2-0.5-0 ppm)
AlerTox Lysozyme 2 ppb 25 ppb (250-125-50-25-0 ppb)
AlerTox Ovalbumin 4 ppb 25 ppb (500-250-100-25-0 ppb)
AlerTox Casein 0.05 ppm 0.20 ppm (5-2.5-1-0.20-0 ppm)
AlerTox Milk 0.05 ppm 0.5 ppm (10-5-2-0.5-0 ppm)
AlerTox BLG 1.5 ppb 10 ppb (300-150-50-10-0 ppb)
AlerTox Crustacean 1 ppb 20 ppb (400-200-80-20-0 ppb)
AlerTox Fish 1.4 ppm 4 ppm (100-50-20-4-0 ppm)

References

Kit 48 wells format 96 wells format
AlerTox Coconut KT-6284 KT-6285
AlerTox Lupine KT-6104 KT-5914
AlerTox Sesame KT-6105 KT-5908
AlerTox Mustard KT-6106 KT-5915
AlerTox Macadamia KT-6286 KT-6287
AlerTox Cashew KT-6107 KT-5916
AlerTox Soy (STI) KT-6108 KT-5906
AlerTox Walnut KT-6109 KT-5909
AlerTox Peanut KT-6110 KT-5905
AlerTox Hazelnut KT-6111 KT-5907
AlerTox Almond KT-6112 KT-5910
AlerTox Pistachio KT-6113 KT-5917
AlerTox Egg KT-6114 KT-5904
AlerTox Lysozyme KT-6115 KT-5757
AlerTox Ovalbumin KT-6116 KT-5759
AlerTox Casein KT-6117 KT-5761
AlerTox Milk KT-6118 KT-5918
AlerTox BLG KT-6119 KT-5919
AlerTox Crustacean KT-6120 KT-5903
AlerTox Fish KT-6121 KT-5920